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il 15  (R&D Systems)


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    Structured Review

    R&D Systems il 15
    Il 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il+15+ab/Goat+IgG+(H%2BL)+Antibody/pmc12278764-160-49-53
    Average 93 stars, based on 199 article reviews
    il 15 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Contribution of astrocyte-derived IL-15 to CD8 T cell effector functions in multiple sclerosis.
    Article Snippet: Flow cytometry analyses were performed using FlowJo (Tree Star, Ashland, OR), and the change in median fluorescence intensity (DMFI) was calculated by subtracting the fluorescence of the isotype from that of the stain. .. Coculture of CD8 T lymphocytes with astrocytes Astrocytes were plated at 1 3 105 cells in 500 ml/well in a 48-well plate and allowed to grow up to ∼70% confluence prior to being activated with cytokines (IL-1b + IFN-g) for 24 h and finally treated with monensin (5 nM) for 1 h. After two washes, blocking anti–IL-15 Ab (20 mg/ml) (clone 34559, R&D Systems) or an isotype control was added for 1 h. The blocking anti–IL-15 mAb (clone 34559) used in our experiments is a well- characterized blocking reagent, as demonstrated by several groups (20, 43– 45). ..

    Article Title: B Cell-Derived IL-15 Enhances CD8 T Cell Cytotoxicity and Is Increased in Multiple Sclerosis Patients
    Article Snippet: .. Conversely, monocytes (1 × 10 5 /well) were activated with LPS (100 pg/ml) and IFN-γ (100 U/ml) for 2 h. Washed B cells or monocytes were subsequently incubated with either blocking anti–IL-15 Ab (20 μg/ml) (R&D Systems) or the isotype control for 1 h as published previously ( 22 ). ..

    Control:

    Article Title: Contribution of astrocyte-derived IL-15 to CD8 T cell effector functions in multiple sclerosis.
    Article Snippet: Flow cytometry analyses were performed using FlowJo (Tree Star, Ashland, OR), and the change in median fluorescence intensity (DMFI) was calculated by subtracting the fluorescence of the isotype from that of the stain. .. Coculture of CD8 T lymphocytes with astrocytes Astrocytes were plated at 1 3 105 cells in 500 ml/well in a 48-well plate and allowed to grow up to ∼70% confluence prior to being activated with cytokines (IL-1b + IFN-g) for 24 h and finally treated with monensin (5 nM) for 1 h. After two washes, blocking anti–IL-15 Ab (20 mg/ml) (clone 34559, R&D Systems) or an isotype control was added for 1 h. The blocking anti–IL-15 mAb (clone 34559) used in our experiments is a well- characterized blocking reagent, as demonstrated by several groups (20, 43– 45). ..

    Article Title: Monocyte surface-bound IL-15 can function as an activating receptor and participate in reverse signaling.
    Article Snippet: .. Briefly, cells were washed three times with PBS (1% FBS and 0.1% NaN3) and incubated in the dark at 4°C with anti-IL-15 Ab (Mab247; R&D Systems) or isotype-matched control Ab (R&D Systems). .. Cells were washed twice and incubated with goat anti-mouse IgG1-PE (Molecular Probes, Eugene, OR).

    Article Title: B Cell-Derived IL-15 Enhances CD8 T Cell Cytotoxicity and Is Increased in Multiple Sclerosis Patients
    Article Snippet: .. Conversely, monocytes (1 × 10 5 /well) were activated with LPS (100 pg/ml) and IFN-γ (100 U/ml) for 2 h. Washed B cells or monocytes were subsequently incubated with either blocking anti–IL-15 Ab (20 μg/ml) (R&D Systems) or the isotype control for 1 h as published previously ( 22 ). ..

    Incubation:

    Article Title: Monocyte surface-bound IL-15 can function as an activating receptor and participate in reverse signaling.
    Article Snippet: .. Briefly, cells were washed three times with PBS (1% FBS and 0.1% NaN3) and incubated in the dark at 4°C with anti-IL-15 Ab (Mab247; R&D Systems) or isotype-matched control Ab (R&D Systems). .. Cells were washed twice and incubated with goat anti-mouse IgG1-PE (Molecular Probes, Eugene, OR).

    Article Title: B Cell-Derived IL-15 Enhances CD8 T Cell Cytotoxicity and Is Increased in Multiple Sclerosis Patients
    Article Snippet: .. Conversely, monocytes (1 × 10 5 /well) were activated with LPS (100 pg/ml) and IFN-γ (100 U/ml) for 2 h. Washed B cells or monocytes were subsequently incubated with either blocking anti–IL-15 Ab (20 μg/ml) (R&D Systems) or the isotype control for 1 h as published previously ( 22 ). ..

    Expressing:

    Article Title: Secretion and Biological Activity of Short Signal Peptide IL-15 Is Chaperoned by IL-15 Receptor Alpha In Vivo
    Article Snippet: The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). .. The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). ..

    Flow Cytometry:

    Article Title: Secretion and Biological Activity of Short Signal Peptide IL-15 Is Chaperoned by IL-15 Receptor Alpha In Vivo
    Article Snippet: The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). .. The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). ..

    Staining:

    Article Title: Secretion and Biological Activity of Short Signal Peptide IL-15 Is Chaperoned by IL-15 Receptor Alpha In Vivo
    Article Snippet: The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). .. The cells were analyzed for IL-15 surface expression by flow cytometry after staining with PE-labeled anti-IL-15 Ab (IC247IP; R&D Systems). ..

    other:

    Article Title: CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation.
    Article Snippet: The following reagents were used: 4-1BB (CD137) IgG1 Fc (838-4B-100; R&D Systems), anti–IL-15 Ab (clone 34559, MAB2471; R&D systems), anti–IL-15RA Ab (clone JM7A4, ab91270; Abcam), anti-CD86 Ab (clone IT2.2, 305410; BioLegend), anti-CD137L Ab (clone 5F4, 311502; BioLegend), anti-CD137 Ab (clone 4B4-1, 309811, BioLegend), and antiCD137 Ab (clone BBK-2, LS-C88297; LifeSpan BioSciences).

    Article Title: STAT3 signaling contributes to the high effector activities of interleukin-15-derived dendritic cells
    Article Snippet: Anti-IL-15Rα Ab (clone eBioJM7A4, FITC) was purchased from eBioscience and anti-IL-15 Ab (clone 34559, PE) was obtained from R&D Systems (Minneapolis, MN, USA).



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